Mouse ACTH ELISA Kit from Innovative Research is a highly sensitive competitive enzyme-linked immunoassay (ELISA) for measuring ACTH in serum, plasma and other biological fluids. Reagents for up to 96 tests.This ELISA Kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this Kit has been pre-coated with Mouse ACTH. During the reaction, Mouse ACTH in samples or Standard competes with a fixed amount of Mouse ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse ACTH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ▒ 2 nm. The concentration of Mouse ACTH in the samples is then determined by comparing the OD of the samples to the standard curve.More Details: Detection Target: ACTH Reactivity: Mouse Range: 15.63 - 1000pg/mL Sensitivity: 9.38pg/mL Storage Conditions: An unopened kit can be stored at 2 - 8 ░C for 1 month unless otherwise specified in the kit documetation. If the kit is not supposed to be used within 1 month, store the components separately upon receipt according to the directions accompanying the kit., At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.For research use only.